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For immunofluorescence, tumor cells grown on poly-lysine-treated coverslides were fixed with 4% paraformaldehyde for 15 min at RT and permeabilized with cold methanol (−20 °C) for 5 min or with 0.1% Triton X-100/PBS at RT for 15 min before incubated overnight at 4 °C with primary antibodies ( Supplementary Table 3 )

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