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The cells were first blocked with 5 μg anti-CD16/CD32 mAb for 5 min, and then stained with 0.5 μg of APC-labeled anti-mouse CD11b mAb, 2 μg of PE-Cy5.5-labeled anti-mouse CD45.2 mAb, 1 μg of Brilliant violet 421-labeled anti-mouse Ly6G, 1 μg Brilliant violet 785-labeled anti-mouse Ly6C, and 2.5 μg Alexa Fluor 488-labeled anti-mouse F4/80 on ice for 10 min
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