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The cell lysate was then subjected to centrifugation at 27,000× g for 40 min, followed by ultracentrifugation at 220,000× g for 1 h at 4 °C to remove the cell debris and the membrane fraction from the lysis solution

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In 1 early study in Mumbai, India, mean serum vitamin B-12 concentration in lacto-vegetarian women was lower than that of nonvegetarian women (71.8 vs