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Full length FIP200 single particles showed an extended density with a C-shaped density at one end, corresponding to the dimeric FIP200NTD previously resolved (Shi et al., 2020)

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Structured Illumination Microscopy was performed using a Zeiss Elyra PS1 microscope using a 63× 1.4 (n.a.) plan apo chromate oil immersion lens and 5 phases and 5 rotations of the illumination pattern