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Hybridization buffer (pH 7.4) contained 10 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA and 10 mM MgCl 2

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In plants exposed to temperature extremes, heavy metal-contaminated soils, drought, or air pollutants, the generation of reactive oxygen species and alterations in the intracellular redox environment perturb cellular physiology (Ogawa, The synthesis of this chemically versatile tripeptide occurs in two ATP-dependent steps (Figure 1