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For conditional MOR knockdown in PVN Glu neurons, we injected the rAAV-CMV-DIO-(EGFP-U6)-shRNA ( Oprm1 )-WPRE-hGH pA (AAV-DIO-shMOR-EGFP) virus or rAAV-CMV-DIO-(EGFP-U6)-shRNA(scramble)-WPRE-hGH polyA (AAV-DIO-EGFP) vector control into the bilateral PVN of VgluT2-Cre mice